2X Universal SYBR Green Fast qPCR Mix from ABclonal Technology

Product Specs
Item2X Universal SYBR Green Fast qPCR Mix
CompanyABclonal Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberRK21203
Quantity5 mL
TypeEnzyme

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ABclonal Technology

500W Cummings Park, Ste. 6500

Woburn, MA 01801

United States

Phone: 888.754.5670
Int’l: +1857.259.4898

Email: service@abclonal.com

Company Profile

Website: https://abclonal.com/

(1) X-box binding protein 1 caused an imbalance in pyroptosis and mitophagy in immature rats with di-(2-ethylhexyl) phthalate-induced testis toxicityGenes & DiseasesMarch 1, 2024Yifan Hong, Xiazhu Zhou, Qi Li, Jing Chen, Yuexin Wei, Chunlan Long, Lianju Shen, Xiangqin Zheng, Dinggang Li, Xia Wang, et al.were stored at −20 °C for subsequent RT‒qPCR. The 2X Universal SYBR Green Fast qPCR Mix (ABclonal, RK21203, China) was used for subsequent qPCR analysis. After qPCR using a CFX96 Touch Real-Time PCR Detection System

(2) Interactome between ASFV and host immune pathway proteinsmSystemsDecember 21, 2023Qijun Wu, Yingying Lei, Ya Zuo, Ji Zhang, Fenglin Guo, Weize Xu, Tanghui Xie, Dang Wang, Guiqing Peng, Xiangru Wang, et al.Wuhan, China). Reverse transcription products were amplified with 2× Universal SYBR Green Fast qPCR Mix (RK21203, ABclonal Biotech Co, Wuhan, China) according to the manufacturer’s instructions. All the reactions

(3) Multi-omics data reveals aberrant gut microbiota-host glycerophospholipid metabolism in association with neuroinflammation in APP/PS1 miceGut MicrobesDecember 18, 2023Xiaohang Qian, Wangxi Hai, Siyue Chen, Miao Zhang, Xufeng Jiang, Huidong Tangexpression of target genes was measured by real-time PCR through 2 × Universal SYBR Green Fast qPCR Mix (RK21203, Abclonal, China). The GAPDH expression level was used as internal reference. The 2(−ΔΔCt) method was

(4) MYOD induced lnc-MEG3 promotes porcine satellite cell differentiation via interacting with DLSTEpigeneticsDecember 1, 2023Liu Q, Li M, Xie S, Tian C, Li J, Wang Y, Li X, Li C.mRNA expression was carried out with 2× Universal SYBR Green FastqPCR Mix (Abclonal, Wuhan, China, Cat#RK21203). The primers associated with qRT-PCR are shown in Tables S1. As previously described, the 2-∆∆CT method

(5) Estradiol-mediated small GTP-binding protein GDP dissociation stimulator induction contributes to sex differences in resilience to ferroptosis in takotsubo syndromeRedox BiologyDecember 1, 2023Ti Wang, Ting Xiong, Yuxue Yang, Xiwei Chen, Ziwei Ma, Bangyun Zuo, Dong Ning, Beibei Zhou, Ruilong Song, Xuesong Liu, et al.quantitative real-time polymerase chain reaction (RT-PCR) on the Applied Biosystems (USA) was performed using SYBR Fast qPCR Mix kit (ABclonal, RK21203, China). The mRNA levels of target genes were normalized to murine GAPDH. The primer sequences used

(6) Epigenetically upregulated NSUN2 confers ferroptosis resistance in endometrial cancer via m5C modification of SLC7A11 mRNARedox BiologyNovember 29, 2023Shuai-Jun Chen, Jun Zhang, Ting Zhou, Shan-Shan Rao, Qian Li, Ling-Yan Xiao, Si-Tian Wei, Hong-Feng ZhangCFX Connect Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) using Universal SYBR Green Fast qPCR Mix (Abclonal, #RK21203). The housekeeping gene GAPDH was used as the internal reference gene for normalization. The primers

(7) Targeting ferroptosis by poly(acrylic) acid coated Mn3O4 nanoparticles alleviates acute liver injuryNature CommunicationsNovember 21, 2023Xinyi Shan, Jiahuan Li, Jiahao Liu, Baoli Feng, Ting Zhang, Qian Liu, Huixin Ma, Honghong Wu, Hao WuReal-time PCR was conducted in a 10-μL reaction mixture by using Universal SYBR Green Fast qPCR Mix (RK21203, ABclonal). Subsequently, the PCR program was run as follows: 95°C, 3 min; 39 cycles (95°C, 5 s; 60

(8) Deficiency of S100A8/A9 attenuates pulmonary microvascular leakage in septic miceRespiratory ResearchNovember 17, 2023Jiang Yu, Boying Zhao, Qiangzhong Pi, Guoxiang Zhou, Zhe Cheng, Can Qu, Xiaowen Wang, Lingwen Kong, Suxin Luo, Dingyuan Du, et al.Real-Time system (Bio-Rad Laboratories, Inc., USA) using 2× Universal SYBR Green Fast qPCR Mix (Cat #RK21203, Abclonal, China). The gene expression levels were normalised to β-actin using the delta-delta Ct (2ΔΔCT)

(9) Role of Kinetochore Scaffold 1 (KNL1) in Tumorigenesis and Tumor Immune Microenvironment in Pan-Cancer: Bioinformatics Analyses and Validation of ExpressionInternational Journal of General MedicineOctober 31, 2023Yixin Ding, Kongjia Wang, Shufen Zhao, Yu Li, Wensheng Qiu, Chunyang Zhu, Yan Wang, Chen Dong, Jiani Liu, Yangyang Lu, et al.37 °C (2 min), 55 °C (15 min), and 85 °C (5 min). 2X Universal SYBR Green Fast qPCR Mix (ABclonal, RK21203) was applied to perform real-time PCR. The qPCR reaction system is as follows: 2X Universal SYBR Green Fast qPCR Mix

(10) USP53 Exerts Tumor-Promoting Effects in Triple-Negative Breast Cancer by Deubiquitinating CRKLCancers (Basel)October 18, 2023Yi Liu, Wei Tang, Feng Yaootherwise, it was considered a high expression. 2.8. Quantitative Real-Time PCR SYBR Green Master Mix (RK21203, Abclonal) was used to perform real-time fluorescence quantitative PCR (qRT-PCR) using an ABI 7500 real-time PCR

(11) TMT-Based Proteomics Reveal the Mechanism of Action of Amygdalin against Rheumatoid Arthritis in a Rat Model through Regulation of Complement and Coagulation CascadesMoleculesOctober 17, 2023Lan Zhou, Jun-Hong Chai, Yi Zhang, Xiao-Jie Jing, Xiang-Wen Kong, Jun Liang, Yong-Gang XiaRK20429, Wuhan, China). qPCR was performed using SYBR Green PCR Master Mix (ABclonal, Catalog number: RK21203, Wuhan, China) on a CFX-384 system. qPCR primers are listed in Table 1. 4.6. TMT-Labeled Quantitative

(12) PRMT6 methylation of STAT3 regulates tumor metastasis in breast cancerCell Death & DiseaseOctober 9, 2023Qianzhi Chen, Qingyi Hu, Yan Chen, Na Shen, Ning Zhang, Anshu Li, Lei Li, Junjun Limanufacturer’s instructions. RT-PCR assays were conducted with 2X Universal SYBR Green Fast qPCR Mix (RK21203, Abclonal) on a QuantStudioTM 3 Real-Time PCR Instrument (Thermo Fisher Scientific). The primer sequences

(13) Chronic stress hinders sensory axon regeneration via impairing mitochondrial cristae and OXPHOSScience AdvancesOctober 6, 2023Yu Ruan, Jin Cheng, Jiafeng Dai, Zhengwen Ma, Shiyu Luo, Run Yan, Lizhao Wang, Jinrui Zhou, Bin Yu, Xiaoping Tong, et al.RK20400). The cDNA was then amplified using the SYBR Green Select Master Mix (ABclonal, catalog no. RK21203) and analyzed on the iCycler real-time PCR Detection System (Bio-Rad). The expression levels were normalized

(14) Adipose METTL14-Elicited N6 -Methyladenosine Promotes Obesity, Insulin Resistance, and NAFLD Through Suppressing β Adrenergic Signaling and LipolysisAdvanced ScienceOctober 1, 2023Qianqian Kang, Xiaorong Zhu, Decheng Ren, Alexander Ky, Ormond A. MacDougald, Robert W. O'Rourke, Liangyou Rui(Alkali Scientific, Pompano Beach, FL), 2× Universal SYBR green fast qPCR mix (ABclonal Technology, Cat# RK21203), Mastercycler qPCR system (Eppendorf), and StepOnePlus RT PCR Systems (Life Technologies Corporation,

(15) Lotus leaf extract can attenuate salpingitis in laying hens by inhibiting apoptosisPoultry ScienceOctober 1, 2023Pupu Yan, Jiali Liu, Yongxi Huang, Yana Li, Jie Yu, Jinjin Xia, Man Liu, Ruonan Bai, Ning Wang, Liwei Guo, et al.sequences (Table 2) were synthesized by Shanghai SANGON bio (Shanghai, China). SYBR Green fast qPCR mix (RK21203; Abclonal, Wuhan, China) was used with the following program: 95°C for 3 min, 30 cycles of 95°C for

(16) All-optical spatiotemporal mapping of ROS dynamics across mitochondrial microdomains in situNature CommunicationsSeptember 27, 2023Shon A. Koren, Nada Ahmed Selim, Lizbeth De la Rosa, Jacob Horn, M. Arsalan Farooqi, Alicia Y. Wei, Annika Müller-Eigner, Jacen Emerson, Gail V. W. Johnson, Andrew P. Wojtovichmanufacturer’s protocol. Real-time PCR was performed using 2x Universal SYBR Green Fast qPCR Mix (Abclonal RK21203) on a CFX Duet Real-Time PCR System (Biorad). Primers are included in Supplementary Table 1. Relative

(17) A comprehensive analysis of the role of QPRT in breast cancerScientific ReportsSeptember 18, 2023Yiqing Yan, Lun Li, Zixin Wang, Jian Pang, Xinyu Guan, Yunchang Yuan, Zhenkun Xia, Wenjun YiII Reverse Transcriptase (Vazyme, R212-01). Then, 2X Universal SYBR Green Fast qPCR Mix (ABclonal, RK21203) was used for qPCR detection. Gene primer sequences used in this study were as follows: QPRT Forwards:

(18) Construction and Validation of a Reliable Disulfidptosis-Related LncRNAs Signature of the Subtype, Prognostic, and Immune Landscape in Colon CancerInt J Mol SciAugust 18, 2023Xiaoqian Dong, Pan Liao, Xiaotong Liu, Zhenni Yang, Yali Wang, Weilong Zhong, Bangmao WangQuantitative PCR analysis was performed using Universal SYBR Green Fast qPCR Mix (ABclonal, Hong Kong, China, RK21203), and the results were calculated with 2(−ΔΔCt) method with the GADPH serving as the internal control

(19) Identification of molecular subtypes based on PANoptosis-related genes and construction of a signature for predicting the prognosis and response to immunotherapy response in hepatocellular carcinomaFrontiers in ImmunologyAugust 18, 2023Jinfeng Zhu, Qian Huang, Xingyu Peng, Chen Luo, Zitao Liu, Dongdong Liu, Huazhao Yuan, Rongfa Yuan, Xuexin Chengwas performed in a LightCycler (Roche, Germany) with 2× Universal SYBR Green Fast qPCR Mix (Abclonal, RK21203). The mRNA expression of LPCAT1 was calculated using the 2−ΔΔCt method. The primer sequences are shown

(20) GSDMA3 deficiency reprograms cellular metabolism and modulates BCR signaling in murine B cellsiScienceAugust 18, 2023Guan F, Luo X, Liu J, Huang Y, Liu Q, Chang J, Fang G, Kang D, Gu H, Luo L, et al.manufacturer’s instructions. Then real-time PCR was performed on qTower 3 (Germamy) with SYBR green (Abclonal, #RK21203) and the following probes: Gapdh, Gsdmd, Il1β, Il18, Caspase 1, Nlrp3, Pkm2, Hk1, Hk3, solute carrier

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6/20/2023Sensitive SYBR Green Reagent by ABclonal Wei Yang SitThe 2X Universal SYBR Green Fast qPCR Mix contains de novel designed universal reference dye, which can realize higher signal resolution and suits for all currently used qPCR instruments (including ... Read Review

2X Universal SYBR Green Fast qPCR Mix from ABclonal Technology

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2X Universal SYBR Green Fast qPCR Mix from ABclonal Technology

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